Clinical Review

Cardiac Biomarkers: Current Standards, Future Trends

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Soluble CD40 Ligand

In the 1980s, postmortem studies confirmed that erosions or ruptures in atherosclerotic fibrous caps lead to platelet activation—the main pathophysiologic contributor in ACS.59 This fundamental actuality suggests that biomarkers of platelet activation may provide supplemental information in patients who present with chest pain of cardiac origin. Another acute inflammatory marker, soluble CD40 ligand, is a marker of active platelet stimulation.60 Increased serum levels of soluble CD40 ligand have been correlated to increased risk for cardiovascular events in apparently healthy women.61

Soluble CD40 ligand, expressed within seconds of platelet activation, is also commonly found on various leukocytes, endothelial cells, and smooth-­muscle cells.60 This may provide insight into cardiovascular disease progression and plaque deterioration that precedes the events of ACS.

Therapeutic antiplatelet medications are now the mainstay in the treatment and prevention of cardiovascular complications associated with ischemic thrombus formation.11 Platelet biomarkers are likely to play an essential supplemental role in the diagnosis of ACS.

Pregnancy-Associated Plasma Protein A

Additional risk-stratifying biomarkers include those that may determine whether a patient has plaques that are acutely vulnerable to rupture. Pregnancy-associated plasma protein A (PAPP-A), first detected in the 1970s in the circulation of pregnant women, is now widely used in first-trimester screening for fetal trisomy.62

Since then, it has been found that PAPP-A, which is theorized to be produced by vascular smooth-muscle cells, is extensively expressed in unstable coronary artery plaques, while minimally expressed in stable plaques.63 Since a significant proportion of patients who present with symptoms of ACS have normal cTn levels, PAPP-A may help identify patients who are at increased risk for subsequent short-term cardiovascular complications resulting from occult disease.64 This relatively new marker may also prove useful for screening in the office setting, identifying outpatients who are at high cardiovascular risk. Further studies are needed to define the release kinetics of PAPP-A, guiding clinicians in its implementation and clinical use.

CONCLUSION

When used in conjunction with the history and physical exam, cardiac biomarkers can provide a simple, noninvasive means to further the clinician’s exploration into a suspected underlying cardiovascular process. As advances continue in the understanding of the pathogenesis of heart disease, new interpretations of existing markers and discovery of novel markers may allow for specific therapeutic interventions to improve patient outcomes.

It is important to note that the list of biomarkers described here is by no means complete, and there is continued interest in finding more specific and sensitive markers of heart disease. Numerous cardiovascular organizations are now suggesting a shift toward a multi-marker strategy to determine the best etiology in the patient who presents with decompensating cardiovascular disease. A change in cardiac enzyme panels may be inevitable in the near future. Practicing PAs and NPs, particularly those who care for patients at risk for CVD, should remain up-to-date and proficient in interpreting those results to help determine the best course of action for each patient.

REFERENCES

1. Thygesen K, Alpert JS, White HD; Joint ESC/ACCF/AHA/WHF Task Force for the Redefinition of Myocardial Infarction. Universal definition of myocardial infarction. Circulation. 2007;116(22):2634-2653.

2. Pearson TA, Mensah GA, Alexander RW, et al. Markers of inflammation and cardiovascular disease: application to clinical and public health practice: a statement for healthcare professionals from the Centers for Disease Control and Prevention and the American Heart Association. Circulation. 2003; 107(3):499-511.

3. Kavsak PA, MacRae AR, Newman AM, et al. Effects of contemporary troponin assay sensitivity on the utility of the early markers myoglobin and CKMB isoforms in evaluating patients with possible acute myocardial infarction. Clin Chim Acta. 2007; 380(1-2):213-216.

4. Eggers KM, Oldgren J, Nordenskjöld A, Lindahl B. Diagnostic value of serial measurement of cardiac markers in patients with chest pain: limited value of adding myoglobin to troponin I for exclusion of myocardial infarction. Am Heart J. 2004; 148(4):574-581.

5. Licka M, Zimmermann R, Zehelein J, et al. Troponin T concentrations 72 hours after myocardial infarction as a serological estimate of infarct size. Heart. 2002;87(6):520-524.

6. Panteghini M, Cuccia C, Bonetti G, et al. Single-point cardiac troponin T at coronary care unit discharge after myocardial infarction correlates with infarct size and ejection fraction. Clin Chem. 2002; 48(9):1432-1436.

7. Giannitsis E, Müller-Bardorff M, Lehrke S, et al. Admission troponin T level predicts clinical outcomes, TIMI flow, and myocardial tissue perfusion after primary percutaneous intervention for acute ST-segment elevation myocardial infarction. Circulation. 2001;104(6):630-635.

8. Rosamond W, Flegal K, Furie K, et al. Heart disease and stroke statistics—2008 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Circulation. 2008;117(4):e25-e146.

9. Lloyd-Jones D, Adams RJ, Brown TM, et al; American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Heart disease and stroke statistics—2010 update: a report from the American Heart Association. Circulation. 2010; 121(7):e46-e215.

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